The Journal of Clinical and Experimental Morphology № 1(9) / 2014

SCIENTIFIC-PRACTICAL REVIEWED JOURNAL
the Research Institute of Human Morphology

CLINICAL MORPHOLOGY

S.V. Vernygorodskyi
Features of structural changes of the gastric mucosa of different age groups patients in hronic gastritis
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FEATURES OF STRUCTURAL CHANGES OF THE GASTRIC MUCOSA OF DIFFERENT AGE GROUPS PATIENTS IN HRONIC GASTRITIS

S.V. Vernygorodskyi

Department of Pathological Anatomy, Forensic Medicine and Law, Vinnitsa National PirogovMemorial Medical University

The structural changes of the gastric mucosa (GM) in different age groups were studied on the basis of pathologic analysis of gastrobiopsies. Prevalence of chronic non-atrophic interstitial gastritis at a young age with moderate dysplasia of the glandular epithelium was established. The obtained results may serve as additional criteria for the differential diagnosis of different morphological types of chronic gastritis.

Key words: gastritis, gastric mucosa, age groups

A.S. Balkanov, V.P. Chernikov, T.A. Belousova, A.M. Kiselev
Morphological signs of the disorders of the blood-brain barrier permeability in brain diffuse glioma
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MORPHOLOGICAL SIGNS OF THE DISORDERS OF THE BLOOD-BRAIN BARRIER PERMEABILITY IN BRAIN DIFFUSE GLIOMA

A.S. Balkanov, V.P. Chernikov, T.A. Belousova, A.M. Kiselev

By method of transmission electron microscopy the ultrastructure of components of the blood-brain barrier in diffuse astrocytoma has been studied on the example of the five biopsy samples, obtained during tumor resection. It has been declared the relative integrity of some components of the capillary wall – tight junctions, basal membrane, pericytes. Signs of endothelium swelling and the presence of the destructive mitochondria in some vessels were observed. Astrocytic shoots in pericapillar zone were swelled in varying degree; it has been viewed as morphological manifestation of the increasing of the blood-brain barrier permeability. The absence of edematous fluid in tumor interstitium suggests, that the swelling of astrocytes shoots compensates in some extent the changes in the barrier permeability, preventing the liquid drain to parenchyma of the tumor and brain. The changes of the endothelial cells mitochondria are discussed as one of the reasons of the blood-brain barrier permeability increasing.

Key words: diffuse astrocytoma, hemocapillar, astrocytes, blood-brain barrier, permeability

EXPERIMENTAL MORPHOLOGY

N.M. Khmelnytskaya, V.V. Kiryanova, A.S. Kirillova, A.B. Veselovsky
Influence of different wavelength narrowband radiation on the skin morphology of laboratory animals
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INFLUENCE OF DIFFERENT WAVELENGTH NARROWBAND RADIATION ON THE SKIN MORPHOLOGY OF LABORATORY ANIMALS

N.M. Khmelnytskaya, V.V. Kiryanova, A.S. Kirillova, A.B. Veselovsky

First studied the influence on the morphological structure of the skin of laboratory animals of narrowband radiation (NR ) with an average wavelength of 650 nm (red light), 540 nm (green light), 470 nm (blue light), 400 nm (violet light) with various doses of experimental light emission . Found that NR with an average wavelength of 650 nm is mainly changing the structure of the dermal collagen framework. All kinds of NR caused increase of mast cells,however, maximum cell response was observed when we use 470 nm wavelength NR with application of stable contact method in continuous mode for 16 minutes (dose 5000 mJ/cm2).

Key words: narrow-band radiation, tissue basophils, dermal collagen framework

V.I. Pivtorak, О.A. Smiyukha
Morphology of Sertoli cells varicocele in experimental and after treatment
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MORPHOLOGY OF SERTOLI CELLS VARICOCELE IN EXPERIMENTAL AND AFTER TREATMENT

V.I. Pivtorak, О.A. Smiyukha

Established anatomical morphometric characteristics of Sertoli cells in the simulation of varicocele and after treatment. After modeling varicocele perimeter and diameter of the cells and their nuclei was significantly decreased with increasing periods of varicocele. After undergoing surgery to Ivanissevich increased performance area, perimeter and diameter of Sertoli cells and their nuclei, but not enough to normalize their function. Forming operation between veins microvascular anastomosis using the method gives better results Sertoli cell regeneration compared to traditional obstructive operations because it normalizes the nuclear-cytoplasmic ratio and thereby creating more favorable conditions for the restoration of spermatogenic function.

Key words: testis, varicocele, modeling, treatment, Sertoli cells

M.V. Kondashevskaya, V.A. Mkhitarov, M.E. Diatroptov
Description of morphofunctional changes in the adrenal cortex in hippocampectomized of the mice F1 (C57BL/6 x DBA/2) under food deprivation
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DESCRIPTION OF MORPHOFUNCTIONAL CHANGES IN THE ADRENAL CORTEX IN HIPPOCAMPECTOMIZED OF THE MICE F1 (C57BL/6 X DBA/2) UNDER FOOD DEPRIVATION

M.V. Kondashevskaya, V.A. Mkhitarov, M.E. Diatroptov

The hippocampus is found to be involved in the regulation of the morphofunctional state of adrenal cortex. The blood level of corticosterone was unchanged after functional load and was increased after hippocampectomy. Food deprivation (23 h, 3 weeks) did not change corticosterone level whereas testosterone decreased in intact mice. After hippocampectomy, testosterone level was unsensitive to the state of the feeding center. The observed hormonal changes were nonspecific with respect to the metabolic signals from the feeding center. The significance of hippocampal inhibitory effect on the hypothalamic-pituitary-adrenal system is discussed.

Key words: mice, hippocampectomy, testosterone, corticosteron, food deprivation, adrenal cortex

E.P. Timokhina, N.V. Yaglova, V.V. Yaglov
Changes in rat spleen morphology and function after different terms of exposure to low doses of DDT
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Changes in rat spleen morphology and function after different terms of  exposure to low doses of DDT

E.P. Timokhina, N.V. Yaglova, V.V. Yaglov

The aim of the study was to evaluate changes in spleen morphology and function after exposure of male Wistar rats to low doses of DDT during 6 and 10 weeks. Exposure to low doses of DDT resulted in decreased size of splenic nodules in account of reduction of marginal zone followed by thinning of mantle zone. Long term exposure of DDT caused dose dependent depletion of germinal centers. Cell death lead to activation of lymphocyte proliferation and formation of new primary splenic nodules. Longer exposure to DDT inhibited splenocyteproliferation and mitogenic response, formation of new nodules, caused enlargement of germinal centers, decrease of red pulp leukocytes content, and increase of megakaryocytepopulation in the spleen.

Key words: DDT, low doses, spleen, morphology, proliferation

E.J. Simonova, A.M. Kosyreva, O.V. Makarova, M.E. Diatroptov
Age changes morfofunktcionalnyj of the immune system in Wistar rats
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AGE CHANGES MORFOFUNKTCIONALNYJ OF THE IMMUNE SYSTEM IN WISTAR RATS

E.J. Simonova, A.M. Kosyreva, O.V. Makarova, M.E. Diatroptov

We studied histological and physiological features of the immune system in newborn, 10-day old and adult male and female Wistar rats. Age-related morphological changes in the immune system in males and females are shown to be similar. They are featured by morphological and functional signs of maturation and activation.

Keywords: age-related changes, immune system, Wistar rats

METHODS

O.V. Paklina, G.R. Setdikova
Operational research methodology material in ductal pancreatic cancer
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OPERATIONAL RESEARCH METHODOLOGY MATERIAL IN DUCTAL PANCREATIC CANCER

O.V. Paklina, G.R. Setdikova

An essential key to successful diagnosis and treatment of cancer, including pancreatic cancer is operating properly cut material for further histological examination.

We present a modern algorithm for morphological study, which is designed to achieve the most objective data on the R- status in different types of pancreatic resections for cancer of the pancreas.

The morphological algorithm is as follows:

1. Orientation resected complex surfaces defining cancer;

2. Determination of patency of the main pancreatic and common bile duct;

3. Dissection resection margins;

4. Marking iron surface;

5. Gross examination;

6. Microscopic and immunohistochemical study using the protocol to enter into an operating histological material in ductal pancreatic cancer.

Key words: pancreatic cancer, ductal adenocarcinoma, organocomplexes dissection, histological sign the protocol

REVIEWS

A.S. Khalansky, L.I. Kondakova, S.A. Gelperina
Transplanted rat glioma 101.8. II. The application in experimental neurooncology and therapy
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TRANSPLANTED RAT GLIOMA 101.8.
II. THE APPLICATION IN EXPERIMENTAL NEUROONCOLOGY AND THERAPY

A.S. Khalansky, L.I. Kondakova, S.A. Gelperina

The review of investigations in the field of experimental neurooncology and preclinical brain tumor therapy, performed on the transplanted rat malignant glioma 101.8 since the time of its appearance in 1968.

E.V. Sheval, Y.R. Musinova
Nuclear envelope: biogenesis mechanisms and pathology development
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NUCLEAR ENVELOPE: BIOGENESIS MECHANISMS AND PATHOLOGY DEVELOPMENT

E.V. Sheval, Y.R. Musinova

The cell nucleus is limited by double-membrane nuclear envelope which provides selective traffic of macromolecules between the nucleus and the cytoplasm and involves in the organization of 3D-architecture of the nucleus, regulation of transcription and replication. Mechanisms of nuclear envelope biogenesis in vivo are poorly understood. Experiments with nuclear envelope protein depletion and over-expression testify in favor of the idea that this structure is not rigid, but may vary even when the individual protein concentrations are changed. Such changes can form the basis for functional plasticity of the structure and the basis of pathological process development.

Key words: nuclear envelope, nuclear pore complexes, endoplasmic reticulum, biogenesis

N.A. Zolotova
Structural and functional characteristics of mucins
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STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF MUCINS

N.A. Zolotova

The surface of epithelial cells of barrier organs, including intestine, is covered by large high glycosylated glycoproteins, called mucins. Some mucins are secreted by epithelial cells and are involved in the formation of mucus layer on the surface of internal organs epithelial lining,others have a transmembrane domain and are included in the glycocalyx. Mucins form a protective barrier of the internal environment, mediate the interaction of epithelial cells with their environment, are involved in cell signaling, they take part in epithelial proliferation, carcinogenesis, immunological reactions. Biophysical properties of mucins are determined by the abundance of O-linked oligosaccharide side chains that make up more than 50% of their molecular weight. Up to 20 different mucin genes have been indentified, MUC1 to MUC22 according to order of their discovery. Mucin genes are expressed in tissue and cell type-specific manner, and their proportion changes during pathology.

Key words: mucins, MUC genes, secretory mucins, transmembrane mucins, glycoproteins

M.N. Boltovskaya, K.A. Artemeva, S.V. Nazimova, I.I. Stepanova, N.A. Starosvetskaya, O.V. Kalyuzhin
Structural and functional comparison of human and mouse placenta as justification for modeling miscarriage
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STRUCTURAL AND FUNCTIONAL COMPARISON OF HUMAN AND MOUSE PLACENTA AS JUSTIFICATION FOR MODELING MISCARRIAGE

M.NBoltovskayaK.AArtemevaS.VNazimovaI.IStepanovaN.AStarosvetskayaO.VKalyuzhin

The comparative characteristics of the development, structure and function of human and mouse placenta, determining the adequacy of mouse models for studying some aspects of the physiology and pathology of pregnancy.

Key words: placenta, human, mouse, morphological and functional features

OBITUARY

In Memory Of Peter Chumachenko
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