CLINICAL AND EXPERIMENTAL MORPHOLOGY № 1(13) / 2015

SCIENTIFIC-PRACTICAL REVIEWED JOURNAL
the Research Institute of Human Morphology

CLINICAL MORPHOLOGY

R S.V. Vernigorodskiy, M.V. Mnikhovich
Precancerous changes of gastric mucosa and Helicobacter pylori infection
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PRECANCEROUS CHANGES OF GASTRIC MUCOSA AND HELICOBACTER PYLORI INFECTION

S.V. Vernigorodskiy, M.V. Mnikhovich

We present the results of follow-up of 98 patients suffered from chronic non-atrophic gastritis and chronic atrophic gastritis with complete and incomplete intestinal metaplasia. We also studied H. pylori infection in groups of patients with precancerous diseases, namely polyps, adenomas, and stomach ulcer. During fibrogastroduodenoscopy, multiple biopsies were performed. The direct relationship was revealed between the frequency of intestinal metaplasia detection, dysplasia and the degree of colonization of the gastric mucosa by H. pylori, which indicated the involvement of the microorganism in the stepwise process of the formation of precancerous changes in the stomach.

Key words: gastric mucosa, intestinal metaplasia, precancerous condition, H. pylori, follow-up, morphological analysis

A.A. Mikhailovskiy, A.A. Kulakov, A.V. Volkov
Maintaining bone tissue volume in the alveolar ridge after symmetric augmentation of extraction sites: clinical and morphological study
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MAINTAINING BONE TISSUE VOLUME IN THE ALVEOLAR RIDGE AFTER SYMMETRIC AUGMENTATION OF EXTRACTION SITES: CLINICAL AND MORPHOLOGICAL STUDY

A.A. Mikhailovskiy, A.A. Kulakov, A.V. Volkov

We studied the effectiveness of osteoplastic material based on deproteinized bovine bone and collagenmembrane for the prevention of bone atrophy after tooth extraction in comparison with the alveolar

healing under blood clot. The study included patients with similar symmetrical teeth to be removed. At the preimplantation stage, teeth extractions were performed at one side with simultaneous filling the extraction sockets with osteoplastic material. The same name symmetrical teeth, after removal of which no materials were used and the healing occurred under blood clot, served as the controls. Volume augmentation of the socket from the examined side was performed using Bio-Oss Collagen material and Bio-Gide collagen membrane. Clinical and histomorphometric analysis revealed the positive impact of the osteoplastic material on the maintaining the bone tissue volume in the extraction sockets. Volume augmentation with Bio-Oss Collagen and bioresorbable membrane Bio Gide that was used as a barrier created favorable conditions for the delayed implantation compared with regeneration under blood clot.

Key words: post-extraction atrophy, osteoplastic materials, collagen membranes

L.M. Mikhaleva, A.E. Birukov, R.V. Plakhov
Clinically and morphologically determined diagnostic algorithm for early gastric cancer
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CLINICALLY AND MORPHOLOGICALLY DETERMINED DIAGNOSTIC ALGORITHM FOR EARLY GASTRIC CANCER

L.M. Mikhaleva, A.E. Birukov, R.V. Plakhov

We present the results of histological examination of early gastric cancer resected by endoscopic submucosal dissection. An algorithm for the morphological study is provided as follows: histology, histochemistry and immunohistochemistry of tissue samples, the procedure of morphological analysis is considered with the findings and conclusions on the effectiveness of endoscopic surgery.

Key words: early gastric cancer, endoscopic submucosal dissection, clinically and morphologically determined diagnostic algorithm

N.V. Mozgaleva, Yu.G. Parkhomenko, O.Yu. Silveystrova, T.S. Skachkova, O.Yu. Shipulina, Yu.Ya. Vengerov
John Cunningham (JC) virus-associated brain damage in HIV infection
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JOHN CUNNINGHAM (JC) VIRUS-ASSOCIATED BRAIN DAMAGE IN HIV INFECTION

N.V. Mozgaleva, Yu.G. Parkhomenko, O.Yu. Silveystrova,T.S. Skachkova, O.Yu. Shipulina, Yu.Ya. Vengerov

Clinical and morphological observation of JC virus-associated brain damage in two HIV-positive patients is presented. In both cases, development of local demyelination and inflammatory changes in the brain were noted with morphological features of opportunistic infections, which was confirmed by polymerase chain reaction (PCR).

Key words: JC virus, HIV infection, brain damage, PCR

A.L. Chernyayev, A.E. Ali-Riza, M.V. Samsonova, T.V. Terskova, S.Yu. Tereshchenko
Acute subtotal invasive pulmonary aspergillosis in 11-year old patient
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ACUTE SUBTOTAL INVASIVE PULMONARY ASPERGILLOSIS IN 11-YEAR OLD PATIENT

A.L. Chernyayev, A.E. Ali-Riza, M.V. Samsonova, T.V. Terskova, S.Yu. Tereshchenko

The article shows the observation of acute subtotal bilateral invasive aspergillosis with a rapidly progressive course that was not diagnosed during life in adolescent with severe immune disorders.

Key words: invasive aspergillosis, lung pathology.

EXPERIMENTAL MORPHOLOGY

A.G. Babaeva, N.M. Gevorkyan, N.V. Tishevskaya, L.L. Golovkina, I.A. Muratova, A.A. Ragimov
Erythropoiesis-stimulating properties of total rna from peripheral blood lymphocytes during erythremia
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ERYTHROPOIESIS-STIMULATING PROPERTIES OF TOTAL RNA FROM PERIPHERAL BLOOD LYMPHOCYTES DURING ERYTHREMIA

A.G. Babaeva, N.M. Gevorkyan, N.V. Tishevskaya, L.L. Golovkina, I.A. Muratova, A.A. Ragimov

We studied the effects of total RNA from peripheral blood lymphocytes of healthy donors and patients with erythremia on the cultures of erythroblastic islands of intact rats and rats with post-transfusion polycythemia. It has been shown that total RNA from the lymphocytes taken from patients with erythremia, in vitro stimulates de novo and de repeto erythropoiesis without xenogeneic restriction.

Key words: total RNA, peripheral blood lymphocytes, erythremia, erythropoiesis, erythroblastic islet, posttransfusion polycythemia, human, rat.

S.V. Saveliev, V.E. Asadchikov, A.V. Buzmakov, D.A. Zolotov, V.I. Gulimova., R.A. Senin
X-ray microtomography and X-ray -fluorescence analysis of human fetal hand during 11-21 weeks of development
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X-RAY MICROTOMOGRAPHY AND X-RAY -FLUORESCENCE ANALYSIS OF HUMAN FETAL HAND DURING 11-21 WEEKS OF DEVELOPMENT

S.V. Saveliev, V.E. Asadchikov, A.V. Buzmakov, D.A. Zolotov, V.I. Gulimova, R.A. Senin

We have studied the differentiation of human fetal hand bones from 11 to 21 weeks of development. It is shown, that the using of X-ray microtomography with different wavelengths is necessary to reveal the details of objects and to create 3D-reconstructions. An independent formation of extracellular matrix elements was discovered. We detected that the accumulation of matrix elements in cartilaginous carpusand metapodium anlages begins some weeks later as compared with differentiating fingers phalanxes and forearm bones. It was established that the fast enlargement of the hand in fetal development occurs mainly at the expense of diaphyses near-epiphyseal zones. Distal areas of phalanxes and nail beds accumulate calcium and phosphorus that leads to delay of their growth.

Key words: development of human hand, X-ray microtomography, X-ray fluorescence analysis, differentiation, ossification

N.V. Yaglova, S.S. Obernikhin
Age-related changes in the number of NK- and NKT‑cells in the thymus of normal mice and in offspring of female mice exposed to immunostimulation in early pregnancy
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AGE-RELATED CHANGES IN THE NUMBER OF NK- AND NKT-CELLS IN THE THYMUS OF NORMAL MICE AND IN OFFSPRING OF FEMALE MICE EXPOSED TO IMMUNOSTIMULATION IN EARLY PREGNANCY

N.V. Yaglova, S.S. Obernikhin

Development of NK cells and NKT cells in the thymus and their abundance during postnatal periods remainunclear. In this study we have examined age-dependent changes in the number of thymic NK cells and NKT cells in normal C57BL/6 mice and in the offspring of females exposed to immunostimulation in early pregnancy. We showed that the time course of the numbers of NK cells and NKT cells in the thymus are normally similar with a maximum at prepubertal period and minimum during puberty. Activation of maternal immune system in early pregnancy before formation of fetal thymus primordium resulted in downregulation of NK1.1 and CD1d expression on thymic lymphocytes and reduction of number of NK- and NKT cells in offspring postnatally. Changes in number of the offspring thymic NK cells, NKT cells and T cells indicate the interdependence of the differentiation of these cell lineages.

Key words: NK cells, NKT cells, T cells, thymus, postnatal development, prenatal exposure

REVIEWS

L.F. Kurilo
Chromosomal diseases of the reproductive organs
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CHROMOSOMAL DISEASES OF THE REPRODUCTIVE ORGANS

L.F. Kurilo

The data (characteristics, etiology, pathogenesis, and structure of chromosomal aberrations) on impaired formation and/or function of the organs of the reproductive system as a result of chromosomal abnormalities (aberrations, CA) (the number or structure of chromosomes) in the body are briefly reviewed.

Key words: chromosomes, chromosome aberrations (anomalies) (CA), numerical CA, structural CA,

reproductive system, gonads, gametes, oogenesis, spermatogenesis, conjugation of homologous parental chromosomes at zygotene of meiotic prophase I, crossing over (exchange of segments between homologous parental chromosomes) at pachytene of meiotic prophase I

T.I. Khomyakova, O.V. Makarova, A.S. Khalansky, V.V. Fedoseeva, L.P. Mikhaylova, Yu.N. Khomyakov
Experimental models of human glioblastoma multiforme
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EXPERIMENTAL MODE LS OF HUMAN GLIOBLASTOMA MULTIFORME

T.I. Khomyakova, O.V. Makarova, A.S. Khalansky, V.V. Fedoseeva, L.P. Mikhaylova, Yu.N. Khomyakov

In the review we present literature data on the experimental models of grade IV astrocytoma/human glioblastoma multiforme. The most common experimental models are described in the terms of their morphological features. The kind of the model can be chosen depending on the goals and objectives of the study. The properties and immunogenicity of the tumor as well as the peculiar properties of the model animals should be considered at the choice of the model.

Key words: glioblastoma multiforme, morphological characteristics, rat, models oncological diseases

ANNIVERSARIES

Valentin V. Yaglov (the 80th anniversary)
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CRITIQUE

Review of the monograph N.V. Yaglova "Syndrome of thyroid diseases"
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