PECULIARITIES OF PRIMARY EPITHELIAL‑LIKE CELL CULTURE FROM BREAST MILK
I.V. Arutyunyan, Y.G. Onishchenko, T.Kh. Fatkhudinov, I.I. Ryumina, A.V. Makarov, A.V. Elchaninov, E.Y. Kananykhina, G.B. Bolshakova
The article presents the study of morphological and functional properties of primary epithelial-like cell culture isolated from human milk. The samples of breast milk were transferred to a culture medium without prior centrifugation. Colonies of epithelial-like cells appeared after 5-7 days of incubation, colonies of fibroblast-like cells and mixed colonies appeared 1-2 weeks later. Fibroblast-like cells, unlike epithelial-like cells, were able to form a tubular net on the surface of the basal membrane matrix. Myoepithelial cells prevailed in primary culture, and 15.3±5.5% cells expressed marker of luminal epithelium cytokeratin 18. Already at passage 0 we observed emergence of multinucleated cells in one of the cultures. These cells expressed Ki67, vimentin, and CD44, 14.6% of them also expressed cytokeratin 18. A mechanism of multinucleated cells formation and their relationship to the possible transformation of mammary epithelial cells requires further studies.
Key words: breast milk, primary culture, epithelial-like cells, polykaryons