the Research Institute of Human Morphology


V.V. Yaglov, N.V. Yaglova
Cell theory as a methodology for studiyng cell biology


The article describes development and the current state of cell theory and methodology for studying cell morphology, metabolism and division as well as formation of tissues and organs based on the ideas of the cell theory.

Key words: cell theory, cell biology, metabolism, regulation, mitosis, meiosis, endomitosis, amitosis


A.V. Asaturova, L.V. Adamyan, L.S. Ezhova
Morphological features of endosalpinx in patients with serous borderline ovarian tumors


The investigation involves 34 patients (72 fallopian tubes) with histologically verified serous borderline ovarian tumors and benign uterine pathology (adenomyosis and / or uterine leiomyoma). In the fallopian tubes of patients with serous borderline tumors epithelial proliferation of the fallopian tubes, the formation of papillary protrusions into the lumen of the fallopian tubes, papillary clusters with the formation of large and small papillary structures, congestion of endosalpingeal plicae, psammoma bodies, inflammation were detected. The analysis of the pathological changes in the fallopian tubes of patients with ovarian serous borderline tumors and comparison them with the existing criteria for the diagnosis were a basis for new algorithm development of papillary tubal hyperplasia diagnosis. With this new algorithm papillary tubal hyperplasia was diagnosed in 73% of fallopian tubes in patients with serous borderline ovarian tumors.

Key words: serous borderline ovarian tumor, papillary endosalpingeal hyperplasia, psammoma bodies, papillary cluster

L.M. Mikhaleva, A.A. Solomatina, M.N. Boltovskaya, E.A. Sadovnikova, N.A. Starosvetskaya, I.I. Stepanova, V.A. Strygina
Сlinical and morphological aspects of ovarian endometriosis depending on the severity of the disease


The aim of the work was to study clinical and morphological aspects of ovarian endometriosis depending on the severity of the disease. Clinical investigation and treatment of 51 patients of reproductive age with ovarian endometriosis was carried out. According to clinical, laboratory and instrumental data, all women were divided into 3 groups: mild, moderate and severe disease. Evaluation of immunohistochemical markers, namely CA-125, glycodelin A and Ki-67, p53, bcl-2, ER, PRA and b-catenin was provided in ovarian tissue; concentration of CA 125 and glycodelin A was recorded in serum and peritoneal fluid. In severe endometriosis CA125 and glycodelin A showed the highest concentrations of serum and peritoneal fluid and high level of expression in endometriotic cyst wall. The expression of Ki-67 and p53 significantly differed in mild and severe endometriosis. The most indicative markers reflecting the severity of endometriosis were CA-125, glycodelin A, Ki-67 and p53.

Key words: ovarian endometriosis, СА 125, glycodelin А, Ki-67, p53, bcl-2, ER, PRA, b-catenin


A.V. Elchaninov, T.Kh. Fatkhudinov, E.Y. Kananykhina, I.V. Arutyunyan, A.V. Makarov, I.G. Charyeva, G.B. Bolshakova, G.T. Sukhikh
Proliferation and cell death in rat liver after subtotal resection


We studied proliferation and cell death in hepatocytes after subtotal resection of the rat liver. We revealed delayed proliferation and temporary blocking of the mitotic cycle. Hepatocyte proliferation proceeded rapidly as indicated by two peaks of the mitotic index. After subtotal resection, we found no additional mechanisms of liver damage such as enhanced synthesis of TNFa causing increased cell death.

Key words: regeneration, liver, hepatocytes, proliferation, apoptosis

A.M. Kosyreva
Age-related immunological and morphological manifestations of system inflammatory response in female Wistar rats


We studied morphological and immunological features of system inflammation response (SIR) one day after intraperitoneal injection of 15 mg/kg of Escherichia coli lipopolysaccharide (LPS) to female Wistar rats. The animals were divided into three age groups as follows: infants (2 days aged rats), prepubertals or pups (10 days old rats) and sexually mature adults (3 month old animals). As compared with newborn and adult females, injection of LPS to prepubertal rats induced more pronounced changes in hepatocytes, increased endotoxin level, lowered corticosterone and estradiol blood concentration. Levels of IFN-γ, TNF-α did not change in 10 day-old rats with SIR, but levels of IL-2 and IL-4 were decreased. In newborn females, minimal inflammatory and alterative changes in the target organs for LPS have been found, namely scarse neutrophils in pulmonary interalveolar septae as well as low-grade vacuolar degeneration and necrosis in the liver. Splenic level of IL-2, IFN-γ and TNF-α in newborn rats with SIR has lowered. Revealed age-related features of SIR in females could be taken into account in the course of the therapy of SIRS and sepsis in women of different age.

Key words: system inflammatory response, female, age-related changes

T.I. Khomyakova, N.A. Zolotova, M.E. Diatroptov, M.A. Diatroptova, S.O. Tsyganova, V.A. Mkhitarov, L.P. Mikhailova, E.A. Postovalova, E.A. Ponomarenko
Effects of intraperitoneal injection of Ultsep during dextran-induced colitis in mice C57BL/6


Immunomodulatory drugs of plant origin are increasingly used in the treatment of chronic inflammatory diseases of the gastrointestinal tract. The aim of the work was to evaluate the therapeutic efficacy of Ultsep at ulcerative colitis in C57Bl /6 mice. Experimental colitis was induced in C57Bl /6 mice administered with aqueous dextran sodium sulfate (DSS) instead of drinking water. Therapeutic efficacy was evaluated by comparing the levels of secretory immunoglobulin A in colonic mucin, serum level of endotoxin and Con A-induced cytokine production as well as by morphological examination of the colon. Treatment of experimental colitis reduced serum endotoxin and increased the level of secretory immunoglobulin A in colonic mucin. The impact on Con A-induced cytokine production by splenocytes was manifested by reduced TNF-α level. Morphological and morphometrical study revealed a statistically significant reduction in inflammattion and ulcerative process in the colon. Conclusion: Ultsep administration effectively reduces inflammation process during dextran-induced colitis in C57Bl /6 mice.

Key words: sodium dextran sulfate, experimental colitis, C57Bl /6 mice, Ultsep, cytokines, endotoxin, secretory immunoglobulin A, morphology

M.S. Khubutiya, K.A. Rogov, Y.A. Zhgutov, A.N. Sklifas, E.V. Klychnikova, E.V. Tazina, M.P. Lebedev, A.A. Temnov
Effect of culture conditions of bone marrow stem cells on the effectiveness of treatment of experimental acute liver failure


The aim of this work was to study the effect of the composition consisting of conditioned medium after culturing mesenchymal stem cells with different percentage of oxygen (21% and 10%) on acetaminophen (paracetamol) – induced acute liver failure (ALF). It was shown that the composition obtained after culturing cells at reduced oxygen content (10%) significantly improves the biochemical parameters and histology of ALF, diminishes the degree of inflammation and stimulates regenerative processes in the liver compared to both the controls and the group of animals treated with the composition obtained after culturing cells at normal oxygen content.

Key words: acute liver failure, stem cells, conditioned medium, acetaminophen

M.B. Chernysheva, I.S. Tsvetkov, G.V. Kozlovskaya, O.V. Makarova
Morphological changes of the colon of Sprague-Dawley rats with obesity


In this work the experimental model of alimentary obesity of mature adult male Sprague-Dawley rats is reproduced. Rats were fed a high fat/carbohydrate diet for 40 weeks. Sprague-Dawley rats with obesity had reactive morphological changes in the colon, which were characterized by a decrease of volume fraction of PAS+ content in goblet cells. The intensity of PAS and alcian blue staining also decreased. The single crypt number of goblet cells showed a tendency to a decrease. The quantity of Bacteroidetes and Akkermansia muciniphila in the mucosal and luminal microbiota, respectively, was observed to decrease. In lamina propria of the colon the quantity of cellular elements and, among them, the relative content of lymphocytes and neutrophils was increased. Additionally the process of collagen formation was intensified in lamina propria.

Key words: alimentary obesity, colon, goblet cells, microbiota, inflammation, rat, male, Sprague-Dawley


S.V. Buravkov
Scanning confocal microscopy of unstained dewaxed paraffin sections


Unstained dewaxed sections of different rat organs (liver, heart, kidney, small intestine and brain) were investigated after routine histological procedure using confocal laser scanning microscopy. The fluorescence was found to reveal the main cell tissue structures without additional staining by histological dyes. The fluorescence spectrum was mostly green although blue fluorescence was found in hepatocytes, lymphocytes, epitheliocytes, endotheliocytes and cardiomyocytes nuclei but not in neurons. The sources of the fluorescence emission are discussed.

Key words: confocal microscopy, unstained paraffin section, autofluorescence, fluorescent microscopy


N.A. Zolotova, O.V. Makarova
Barrier function of colonic mucins in health and ulcerative colitis


Mucins are highly glycosylated glycoproteins secreted by epithelial cells. Colonic mucins form two primary barriers: mucus and glycocalyx that protect internal environment against microbial pathogens and harmful agents. Impairements of the structure and function of these barriers plays an important role in the pathogenesis of ulcerative colitis.

Key words: mucins, mucus, glycocalyx, colon, ulcerative colitis


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