Endometriosis is one of the most common gynecological diseases and is diagnosed in 10% of women of
reproductive age. Severe clinical manifestations and a recurring course of the disease have a negative impact
on the quality of life and work capacity of women.
Patients underwent operative treatment with laparoscopic access in the volume of cystectomy using the tools
of Karl Storz Company with the subsequent histological examination of excised endometrioid formations.
Immunohistochemical study was carried out with immunohistosteiner Bond-max (Leica, Germany) using
monoclonal antibodies (DAKO, UK, Lab Vision, USA) against receptors of estrogen and progesterone,
marker of proliferative activity Ki-67, inhibitor of apoptosis bcl- 2, pro-apoptotic proteinp53, intercellular
adhesion protein β-katenin. The selection of antigens was based on the evaluation of their functional activity
both under the control of the fundamental processes of proliferative activity, apoptosis, cell-cell adhesion,
and in relation to more specific mechanisms, in particular participation in the estrogen and progesteronemediated
signaling cascades. For statistical processing of the data obtained, the computer program Statistica
10 (StatSoft Inc., USA) was used. The level of statistical significance was accepted as p<0,05.
According to results of immunohistochemical staining, the expression of ERβ was 33,33±4,32% in recurrent
endometriosis, which was significantly higher than in samples obtained from newly diagnosed endometriosis,
20,71±4,92% (p<0,05). On the contrary, in evaluating the expression of progesterone receptors the value
of the indicator was statistically significantly higher in Group II (30,56±3,92% vs 55,71±9,68%, p=0,045).
The level of the expression of the apoptosis inhibitor bcl-2 was higher in group I reaching 59,09±5,09%
(p=0,039). The expression of proapoptotic protein p53 was almost 4 times less than in the group of recurrence
endometriosis (4,17±1,40% vs 19,29±1,84%, p=0,048). In group 1, there was a significant increase
in the expression of Ki-67 and β-catenin (25,83±3,95% vs 12,14±4,06%, p=0,042 and 51,67±4,14% vs
38,57±4,82%, p=0,039, respectively).
Immunohistochemical study revealed significant differences in the expression of the indicators of proliferation,
apoptosis and intercellullar adhesion (p<0,05) in recurrent ovarian endometriosis, which will subsequently
develop a differentiated approach to the management of patients.
Key words: recurrent ovarian endometriosis, immunohistochemical indicators, Ki-67, p53, bcl-2, ER, PRA,