the Research Institute of Human Morphology

Clinical morphology

Yu.R. Ziuzia, M.G. Kuzina, Yu.G. Parkhomenko
Morphological features of mycobacteriosises caused by nontuberculous mycobacteria


A comparative morphological study was carried out on mycobacteriosises caused by nontuberculous mycobacteria.
The study was based on autopsy, biopsy and resection material from 31 patients with non-HIVassociated
nontuberculous mycobacteriosis (group 1) and 104 patients with HIV-associated nontuberculous
mycobacteriosis (group 2). Steady increase in figures of both HIV-associated and non-HIV-associated nontuberculous
mycobacteriosis cases was observed in recent years. Differences associated with sex and age
of patients, species spectrum of mycobacteria and forms of mycobacteriosis were detected. Morphological
distinctions from tuberculous mycobacteriosis manifested in predominance of macrophage and giant cell
reaction were noticed, particularly in case of HIV-associated nontuberculous mycobacteriosis.

Key words: nontuberculous mycobacteriosis, AIDS-related opportunistic infections, lungs

L.M. Mikhaleva, A.A. Solomatina, M.A. Kurtzer, V.A. Strygina, E.A. Sadovnikova
Immunohistochemical phenotype of recurrent ovarian endometriomas


Endometriosis is one of the most common gynecological diseases and is diagnosed in 10% of women of
reproductive age. Severe clinical manifestations and a recurring course of the disease have a negative impact
on the quality of life and work capacity of women.
Patients underwent operative treatment with laparoscopic access in the volume of cystectomy using the tools
of Karl Storz Company with the subsequent histological examination of excised endometrioid formations.
Immunohistochemical study was carried out with immunohistosteiner Bond-max (Leica, Germany) using
monoclonal antibodies (DAKO, UK, Lab Vision, USA) against receptors of estrogen and progesterone,
marker of proliferative activity Ki-67, inhibitor of apoptosis bcl- 2, pro-apoptotic proteinp53, intercellular
adhesion protein β-katenin. The selection of antigens was based on the evaluation of their functional activity
both under the control of the fundamental processes of proliferative activity, apoptosis, cell-cell adhesion,
and in relation to more specific mechanisms, in particular participation in the estrogen and progesteronemediated
signaling cascades. For statistical processing of the data obtained, the computer program Statistica
10 (StatSoft Inc., USA) was used. The level of statistical significance was accepted as p<0,05.
According to results of immunohistochemical staining, the expression of ERβ was 33,33±4,32% in recurrent
endometriosis, which was significantly higher than in samples obtained from newly diagnosed endometriosis,
20,71±4,92% (p<0,05). On the contrary, in evaluating the expression of progesterone receptors the value
of the indicator was statistically significantly higher in Group II (30,56±3,92% vs 55,71±9,68%, p=0,045).
The level of the expression of the apoptosis inhibitor bcl-2 was higher in group I reaching 59,09±5,09%
(p=0,039). The expression of proapoptotic protein p53 was almost 4 times less than in the group of recurrence
endometriosis (4,17±1,40% vs 19,29±1,84%, p=0,048). In group 1, there was a significant increase
in the expression of Ki-67 and β-catenin (25,83±3,95% vs 12,14±4,06%, p=0,042 and 51,67±4,14% vs
38,57±4,82%, p=0,039, respectively).
Immunohistochemical study revealed significant differences in the expression of the indicators of proliferation,
apoptosis and intercellullar adhesion (p<0,05) in recurrent ovarian endometriosis, which will subsequently
develop a differentiated approach to the management of patients.

Key words: recurrent ovarian endometriosis, immunohistochemical indicators, Ki-67, p53, bcl-2, ER, PRA,

A.A. Kanibolotskiy, P.O. Svishcheva, O.V. Makarova, L.V. Kaktursky
Hydatid ovarian cyst (clinical and morphological observation)


We describe a clinical case of hydatid ovarian cyst in 32-year-old infertile patient S. with a history of
hydatid cyst liver. She was admitted to the hospital for surgical treatment with a diagnosis of left ovarian
cystadenoma. The hydatid cyst was removed during surgery, the diagnosis was confirmed morphologically.
Both ovaries were resected due to cysts in a cesarean section two years after the removal of the hydatid cyst.
Morphological examination revealed luteal cysts in the ovaries, no signs of echinococcosis were found. The
article deals with the epidemiology, clinical picture, morphological features of echinococcosis associated
with ovarian injury.

Key words: echinococcus, hydatid disease, hydatid ovarian cyst, hydatidosis

V.N. Salkov, R.M. Khudoerkov, D.N. Voronkov, M.V. Ivanov
Morphometry of glial cells in the human substantia nigra in ageing and Parkinson disease


The purpose of the study was to evaluate the quantitative characteristics of astrocytes containing glia-specific
proteins in the autopsy samples of human substantia nigra in ageing and parkinson disease (PD). Glial fibrillary
acidic protein (GFAP), S100 protein and glutamine synthetase were immunohistochemically identified
in the substantia nigra of mature and elderly persons (control), senile patients as well as patients with PD.
The distribution of astrocytes including the number of the cells per1mm2 in different regions of substantia
nigra was studied using computer morphometry. It was found that the number of GFAP-positive and S100-
positive astrocytes in senile persons and patients with PD was more in reticular part of substantia nigra than
in the compact part. These indices in the compact and in the reticular parts of substantia nigra were higher
in patients with BP compared with the controls. The number of astrocytes in substantia nigra did not change
significantly during the ageing process, but in patients with PD the quantity of GFAP-positive and S100-
positive cells was increased. In patients with PD, activated astrocytes showed increased GFAP expression
and altered morphology of their processes.

Key words: human brain, substantia nigra, Parkinson disease, ageing, neuroglia, astrocytes

M.V. Samsonova, A.L. Chernyaev, S.A. Krasovsky, E.L. Amelina, M.V. Usacheva
Pathology of cystic fibrosis in adult patients


In this study, pathology of cystic fibrosis in adults was analyzed. It was shown that pathological changes
were multivarious including multiple festering bronchiectasis, polysegmental pneumonia, peribronchial and
interstitial fibrosis, irregular and/or bullous emphysema. The relationship between the severity of the mutation and pathological changes in the lungs was not revealed whereas pancreatic involvement in the process
depends on mutation variant. Typical cystic fibrosis of the pancreas occurs in only one third of adult patients
with cystic fibrosis, and pulmonary heart disease, in 56.5% of cases.

Key words: cystic fibrosis, pathology, bronchiectasis, cor pulmonale

I.A. Shupletsova, A.M. Kovrigina, T.N. Moiseeva
Syncytial variant of nodular lymphocyte predominant Нodgkin lymphoma


The article describes two cases of nodular lymphocyte predominant Hodgkin lymphoma with syncytial
growth pattern of tumor cells. It is extremely rare for this variant of Hodgkin lymphoma to occur.
Immunohistochemical study using a wide panel of antibodies revealed that tumor cells maintain the B-cell
transcriptional program expressing CD20, BCL-6 and showing intense reaction for B-cell transcription factors
PAX5 and Oct-2. The article reports different clinical course of two cases of syncytial variant of nodular
lymphocyte predominant Hodgkin lymphoma. The issues of correlation with tumoral immunophenotype and
reactive microenvironment as well as the problems of diagnosing nodular lymphocyte-predominant Hodgkin
lymphoma from other variants of lymphomas are discussed.

Key words: nodular lymphocyte predominant Hodgkin lymphoma, syncytial variant, immunophenotype

Experimental morphology

E.Yu. Kananykhina, F.S. Rusanov, G.B. Bolshakova
Morphofunctional features in the formation of scar tissue in the healing of wounds of various locations in rats


We studied location-dependent morphological characteristics of healing skin wounds based on our previously
developed model of excision injury to the dorsal and abdominal skin in Wistar rats. We revealed pronounced
differences in the nature of inflammation and scar formation, namely faster reduction of macrophage infiltration,
more abundant active fibroblasts and earlier onset of collagen synthesis in abdominal wounds. The tensile
strength of abdominal scars was greater compared with dorsal scars on post-operative day 30, which may
be due to a higher ratio of type I collagen to type III collagen and the presence of elastin not characteristic
of the scar tissue in the back. Further study of molecular mechanisms underlying the healing of differently
located wounds will approach the targeted regulation of the processes of inflammation and collagen synthesis.

Key words: full-thickness skin wound, experimental model, abdomen, back, regeneration, scar, tear strength,
collagen type I, collagen type III, elastin, FAP-α, CD-68

D.B. Nikityuk, S.V. Klochkova, N.T. Alekseeva, A.G. Kvaratskheliya, V.A. Tutelyan
Morphological features of the murine mesenteric lymph nodes under the action of low radiation doses in experimental space flight


Morphological features of mesenteric lymph nodes in mice under the influence of low radiation doses under
experimental conditions of space flight were studied.
Materials and methods. The objects of study were the mesenteric lymph nodes of male F1 mice (CBAxC57BL6)
exposed to space flight factors. A long-term exposure to gamma radiation was simulated (the dose rate of
the source was 25 cGy per hour, and the maximum irradiation dose was 350 cGy). Transverse histological
sections were stained with hematoxylin and eosin, by van Gieson’s method and according to Grimelius’s
silvering method. In the course of the study, structural changes in the mesenteric lymph nodes in mice were
determined, and their quantification was determined depending on the dose and the time of irradiation as
well as from the terms of the rehabilitation period after the radiation exposure. Studies have shown that the
murine lymph nodes are characterized by a sufficiently high sensitivity to the action of the radiation factor.
The terms of restoration of lymphoid tissue in the mesenteric lymph nodes after the end of the radiation
exposure are revealed.

Key words: mesenteric lymph nodes, space flight factors, rehabilitation period


N.S. Aleksandrov, S.T. Avraamova, U.A. Kirillov, T.O. Babicheva, V.I. Kukushkin, D.N. Artemyev
Application of raman-fluorescence spectroscopy for diagnostics of clear cell renal cell carcinoma


To assess the possibilities of using Raman and fluorescence spectroscopy as a diagnostic tool for pre- and
intraoperative identification of the clear cell variant of renal cell carcinoma (CCRCC), tumor samples were
obtained from various parts of the initial tumor as well as from the kidneys of the patients in control group
without clinical and morphological signs and manifestations of renal pathology. As a result of the study,
specific spectral markers were detected in each group. Identification of samples from control groups and
kidney cancer was performed using projection to latent structures method and discriminant analysis that
provided 98% degree of accuracy in separating while the sensitivity and specificity of the method were
found 87,5% and 100%, respectively.

Key words: diagnostics, renal cell carcinoma, Raman-fluorescence spectroscopy

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